Darwin RNA-seq

Complete RNA-seq analysis: QC, trimming, alignment, quantification, and differential expression Steps: Step 1: FastQC (raw reads); Step 2: Trim Galore; Step 3: STAR Alignment; Step 4: Salmon Quantification; Step 5: SAMtools Sort & Index; Step 6: FastQC (trimmed reads); Step 7: DESeq2 Differential Expression; Step 8: MultiQC Report. Verticals: transcriptomics, cardiac_genomics, oncogenomics, neuroscience, immunogenomics.

Use in DarwinOmics

How to use it

Use this workflow element when the submitted analysis needs a multi-step execution path, imported workflow, Darwinomics wrapper, or workflow-scale comparison rather than a single module.